EPG waveform library for Graphocephala atropunctata (Hemiptera: Cicadellidae): Effect of adhesive, input resistor, and voltage levels on waveform appearance and stylet probing behaviors.
Identifieur interne : 000212 ( Main/Exploration ); précédent : 000211; suivant : 000213EPG waveform library for Graphocephala atropunctata (Hemiptera: Cicadellidae): Effect of adhesive, input resistor, and voltage levels on waveform appearance and stylet probing behaviors.
Auteurs : Felix A. Cervantes [États-Unis] ; Elaine A. Backus [États-Unis]Source :
- Journal of insect physiology [ 1879-1611 ]
Descripteurs français
- KwdFr :
- MESH :
- méthodes : Électrophysiologie.
- parasitologie : Vitis.
- physiologie : Comportement alimentaire, Hemiptera.
- Adhésifs, Animaux, Bouche, Électrophysiologie.
English descriptors
- KwdEn :
- MESH :
- chemical : Adhesives.
- instrumentation : Electrophysiology.
- methods : Electrophysiology.
- parasitology : Vitis.
- physiology : Feeding Behavior, Hemiptera.
- Animals, Mouth.
Abstract
Blue-green sharpshooter, Graphocephala atropunctata, is a native California vector of Xylella fastidiosa (Xf), a foregut-borne bacterium that is the causal agent of Pierce's disease in grapevines. A 3rd-generation, AC-DC electropenetrograph (EPG monitor) was used to record stylet probing and ingestion behaviors of adult G. atropunctata on healthy grapevines. This study presents for the first time a complete, updated waveform library for this species, as well as effects of different electropenetrograph settings and adhesives on waveform appearances. Both AC and DC applied signals were used with input resistor (Ri) levels (amplifier sensitivities) of 106, 107, 108 and 109 Ohms, as well as two type of adhesives, conducting silver paint and handmade silver glue. Waveform description, characterization of electrical origins (R versus emf components), and proposed biological meanings of waveforms are reported, as well as qualitative differences in waveform appearances observed with different electropenetrograph settings and adhesives. In addition, a quantitative study with AC signal, using two applied voltage levels (50 and 200 mV) and two Ri levels (107 and 109 Ohms) was performed. Intermediate Ri levels 107 and 108 Ohms provided EPG waveforms with the greatest amount of information, because both levels captured similar proportions of R and emf components, as supported by appearance, clarity, and definition of waveforms. Similarly, use of a gold wire loop plus handmade silver glue provided more definition of waveforms than a gold wire loop plus commercial conducting silver paint. Qualitative/observational evidence suggested that AC applied signal caused fewer aberrant behaviors/waveforms than DC applied signal. In the quantitative study, behavioral components of the sharpshooter X wave were the most affected by changes in Ri and voltage level. Because the X wave probably represents X. fastidiosa inoculation behavior, future studies of X. fastidiosa inoculation via EPG will require carefully determined instrument settings. An intermediate Ri level such as 108 Ohms with low voltage, AC applied signal, and gold wire loop plus silver glue is recommended as the best electropenetrograph methods to conduct future EPG studies of sharpshooter inoculation behaviors on Xf-resistant and -susceptible grapevine.
DOI: 10.1016/j.jinsphys.2018.05.008
PubMed: 29859838
Affiliations:
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Cervantes</name><affiliation wicri:level="2"><nlm:affiliation>USDA Agricultural Research Service, San Joaquin Valley Agricultural Sciences Center, 9611 South Riverbend Ave., Parlier, CA 93648-9757, United States.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>USDA Agricultural Research Service, San Joaquin Valley Agricultural Sciences Center, 9611 South Riverbend Ave., Parlier, CA 93648-9757</wicri:regionArea><placeName><region type="state">Californie</region></placeName></affiliation></author><author><name sortKey="Backus, Elaine A" sort="Backus, Elaine A" uniqKey="Backus E" first="Elaine A" last="Backus">Elaine A. Backus</name><affiliation wicri:level="2"><nlm:affiliation>USDA Agricultural Research Service, San Joaquin Valley Agricultural Sciences Center, 9611 South Riverbend Ave., Parlier, CA 93648-9757, United States. 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Cervantes</name><affiliation wicri:level="2"><nlm:affiliation>USDA Agricultural Research Service, San Joaquin Valley Agricultural Sciences Center, 9611 South Riverbend Ave., Parlier, CA 93648-9757, United States.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>USDA Agricultural Research Service, San Joaquin Valley Agricultural Sciences Center, 9611 South Riverbend Ave., Parlier, CA 93648-9757</wicri:regionArea><placeName><region type="state">Californie</region></placeName></affiliation></author><author><name sortKey="Backus, Elaine A" sort="Backus, Elaine A" uniqKey="Backus E" first="Elaine A" last="Backus">Elaine A. Backus</name><affiliation wicri:level="2"><nlm:affiliation>USDA Agricultural Research Service, San Joaquin Valley Agricultural Sciences Center, 9611 South Riverbend Ave., Parlier, CA 93648-9757, United States. Electronic address: elaine.backus@ars.usda.gov.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>USDA Agricultural Research Service, San Joaquin Valley Agricultural Sciences Center, 9611 South Riverbend Ave., Parlier, CA 93648-9757</wicri:regionArea><placeName><region type="state">Californie</region></placeName></affiliation></author></analytic><series><title level="j">Journal of insect physiology</title><idno type="eISSN">1879-1611</idno></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Adhesives (MeSH)</term><term>Animals (MeSH)</term><term>Electrophysiology (instrumentation)</term><term>Electrophysiology (methods)</term><term>Feeding Behavior (physiology)</term><term>Hemiptera (physiology)</term><term>Mouth (MeSH)</term><term>Vitis (parasitology)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Adhésifs (MeSH)</term><term>Animaux (MeSH)</term><term>Bouche (MeSH)</term><term>Comportement alimentaire (physiologie)</term><term>Hemiptera (physiologie)</term><term>Vitis (parasitologie)</term><term>Électrophysiologie (instrumentation)</term><term>Électrophysiologie (méthodes)</term></keywords><keywords scheme="MESH" type="chemical" xml:lang="en"><term>Adhesives</term></keywords><keywords scheme="MESH" qualifier="instrumentation" xml:lang="en"><term>Electrophysiology</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Electrophysiology</term></keywords><keywords scheme="MESH" qualifier="méthodes" xml:lang="fr"><term>Électrophysiologie</term></keywords><keywords scheme="MESH" qualifier="parasitologie" xml:lang="fr"><term>Vitis</term></keywords><keywords scheme="MESH" qualifier="parasitology" xml:lang="en"><term>Vitis</term></keywords><keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Comportement alimentaire</term><term>Hemiptera</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Feeding Behavior</term><term>Hemiptera</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Mouth</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Adhésifs</term><term>Animaux</term><term>Bouche</term><term>Électrophysiologie</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Blue-green sharpshooter, Graphocephala atropunctata, is a native California vector of Xylella fastidiosa (Xf), a foregut-borne bacterium that is the causal agent of Pierce's disease in grapevines. A 3rd-generation, AC-DC electropenetrograph (EPG monitor) was used to record stylet probing and ingestion behaviors of adult G. atropunctata on healthy grapevines. This study presents for the first time a complete, updated waveform library for this species, as well as effects of different electropenetrograph settings and adhesives on waveform appearances. Both AC and DC applied signals were used with input resistor (Ri) levels (amplifier sensitivities) of 10<sup>6</sup>, 10<sup>7</sup>, 10<sup>8</sup> and 10<sup>9</sup> Ohms, as well as two type of adhesives, conducting silver paint and handmade silver glue. Waveform description, characterization of electrical origins (R versus emf components), and proposed biological meanings of waveforms are reported, as well as qualitative differences in waveform appearances observed with different electropenetrograph settings and adhesives. In addition, a quantitative study with AC signal, using two applied voltage levels (50 and 200 mV) and two Ri levels (10<sup>7</sup> and 10<sup>9</sup> Ohms) was performed. Intermediate Ri levels 10<sup>7</sup> and 10<sup>8</sup> Ohms provided EPG waveforms with the greatest amount of information, because both levels captured similar proportions of R and emf components, as supported by appearance, clarity, and definition of waveforms. Similarly, use of a gold wire loop plus handmade silver glue provided more definition of waveforms than a gold wire loop plus commercial conducting silver paint. Qualitative/observational evidence suggested that AC applied signal caused fewer aberrant behaviors/waveforms than DC applied signal. In the quantitative study, behavioral components of the sharpshooter X wave were the most affected by changes in Ri and voltage level. Because the X wave probably represents X. fastidiosa inoculation behavior, future studies of X. fastidiosa inoculation via EPG will require carefully determined instrument settings. An intermediate Ri level such as 10<sup>8</sup> Ohms with low voltage, AC applied signal, and gold wire loop plus silver glue is recommended as the best electropenetrograph methods to conduct future EPG studies of sharpshooter inoculation behaviors on Xf-resistant and -susceptible grapevine.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">29859838</PMID><DateCompleted><Year>2019</Year><Month>09</Month><Day>30</Day></DateCompleted><DateRevised><Year>2019</Year><Month>09</Month><Day>30</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1879-1611</ISSN><JournalIssue CitedMedium="Internet"><Volume>109</Volume><PubDate><MedlineDate>2018 Aug - Sep</MedlineDate></PubDate></JournalIssue><Title>Journal of insect physiology</Title><ISOAbbreviation>J Insect Physiol</ISOAbbreviation></Journal><ArticleTitle>EPG waveform library for Graphocephala atropunctata (Hemiptera: Cicadellidae): Effect of adhesive, input resistor, and voltage levels on waveform appearance and stylet probing behaviors.</ArticleTitle><Pagination><MedlinePgn>21-40</MedlinePgn></Pagination><ELocationID EIdType="pii" ValidYN="Y">S0022-1910(18)30020-9</ELocationID><ELocationID EIdType="doi" ValidYN="Y">10.1016/j.jinsphys.2018.05.008</ELocationID><Abstract><AbstractText>Blue-green sharpshooter, Graphocephala atropunctata, is a native California vector of Xylella fastidiosa (Xf), a foregut-borne bacterium that is the causal agent of Pierce's disease in grapevines. A 3rd-generation, AC-DC electropenetrograph (EPG monitor) was used to record stylet probing and ingestion behaviors of adult G. atropunctata on healthy grapevines. This study presents for the first time a complete, updated waveform library for this species, as well as effects of different electropenetrograph settings and adhesives on waveform appearances. Both AC and DC applied signals were used with input resistor (Ri) levels (amplifier sensitivities) of 10<sup>6</sup>, 10<sup>7</sup>, 10<sup>8</sup> and 10<sup>9</sup> Ohms, as well as two type of adhesives, conducting silver paint and handmade silver glue. Waveform description, characterization of electrical origins (R versus emf components), and proposed biological meanings of waveforms are reported, as well as qualitative differences in waveform appearances observed with different electropenetrograph settings and adhesives. In addition, a quantitative study with AC signal, using two applied voltage levels (50 and 200 mV) and two Ri levels (10<sup>7</sup> and 10<sup>9</sup> Ohms) was performed. Intermediate Ri levels 10<sup>7</sup> and 10<sup>8</sup> Ohms provided EPG waveforms with the greatest amount of information, because both levels captured similar proportions of R and emf components, as supported by appearance, clarity, and definition of waveforms. Similarly, use of a gold wire loop plus handmade silver glue provided more definition of waveforms than a gold wire loop plus commercial conducting silver paint. Qualitative/observational evidence suggested that AC applied signal caused fewer aberrant behaviors/waveforms than DC applied signal. In the quantitative study, behavioral components of the sharpshooter X wave were the most affected by changes in Ri and voltage level. Because the X wave probably represents X. fastidiosa inoculation behavior, future studies of X. fastidiosa inoculation via EPG will require carefully determined instrument settings. An intermediate Ri level such as 10<sup>8</sup> Ohms with low voltage, AC applied signal, and gold wire loop plus silver glue is recommended as the best electropenetrograph methods to conduct future EPG studies of sharpshooter inoculation behaviors on Xf-resistant and -susceptible grapevine.</AbstractText><CopyrightInformation>Copyright © 2018. Published by Elsevier Ltd.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Cervantes</LastName><ForeName>Felix A</ForeName><Initials>FA</Initials><AffiliationInfo><Affiliation>USDA Agricultural Research Service, San Joaquin Valley Agricultural Sciences Center, 9611 South Riverbend Ave., Parlier, CA 93648-9757, United States.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Backus</LastName><ForeName>Elaine A</ForeName><Initials>EA</Initials><AffiliationInfo><Affiliation>USDA Agricultural Research Service, San Joaquin Valley Agricultural Sciences Center, 9611 South Riverbend Ave., Parlier, CA 93648-9757, United States. Electronic address: elaine.backus@ars.usda.gov.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013486">Research Support, U.S. Gov't, Non-P.H.S.</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2018</Year><Month>05</Month><Day>31</Day></ArticleDate></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>J Insect Physiol</MedlineTA><NlmUniqueID>2985080R</NlmUniqueID><ISSNLinking>0022-1910</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D000269">Adhesives</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000269" MajorTopicYN="N">Adhesives</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D000818" MajorTopicYN="N">Animals</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D004594" MajorTopicYN="N">Electrophysiology</DescriptorName><QualifierName UI="Q000295" MajorTopicYN="N">instrumentation</QualifierName><QualifierName UI="Q000379" MajorTopicYN="Y">methods</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005247" MajorTopicYN="N">Feeding Behavior</DescriptorName><QualifierName UI="Q000502" MajorTopicYN="Y">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D006430" MajorTopicYN="N">Hemiptera</DescriptorName><QualifierName UI="Q000502" MajorTopicYN="Y">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D009055" MajorTopicYN="N">Mouth</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D027843" MajorTopicYN="N">Vitis</DescriptorName><QualifierName UI="Q000469" MajorTopicYN="N">parasitology</QualifierName></MeshHeading></MeshHeadingList><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="Y">Applied signal</Keyword><Keyword MajorTopicYN="Y">EPG</Keyword><Keyword MajorTopicYN="Y">Electrical penetration graph</Keyword><Keyword MajorTopicYN="Y">Feeding behavior</Keyword><Keyword MajorTopicYN="Y">Stylet penetration</Keyword><Keyword MajorTopicYN="Y">Xylem ingestion</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2018</Year><Month>01</Month><Day>19</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2018</Year><Month>05</Month><Day>23</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2018</Year><Month>05</Month><Day>23</Day></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2018</Year><Month>6</Month><Day>4</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2019</Year><Month>10</Month><Day>1</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2018</Year><Month>6</Month><Day>4</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">29859838</ArticleId><ArticleId IdType="pii">S0022-1910(18)30020-9</ArticleId><ArticleId IdType="doi">10.1016/j.jinsphys.2018.05.008</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>États-Unis</li></country><region><li>Californie</li></region></list><tree><country name="États-Unis"><region name="Californie"><name sortKey="Cervantes, Felix A" sort="Cervantes, Felix A" uniqKey="Cervantes F" first="Felix A" last="Cervantes">Felix A. Cervantes</name></region><name sortKey="Backus, Elaine A" sort="Backus, Elaine A" uniqKey="Backus E" first="Elaine A" last="Backus">Elaine A. Backus</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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